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调制荧光成像系统 M系列IMAGING-PAM
  • 调制荧光成像系统 M系列IMAGING-PAM
  • 调制荧光成像系统 M系列IMAGING-PAM

    产品报价: 面议
    品  牌: WALZ
    产品型号: ZQ-WALZ001 (IMAGING-PAM )
    所在地区: 上海
    (联系我时,请说明是在 教育装备采购网上看到的,谢谢!)
    详细说明

    M系列调制叶绿素荧光成像系统IMAGING-PAM

    简便、快速、可靠、对样品无干扰
    代表了调制荧光技术的未来发展方向!
    Schreiber教授因发明PAM系列调制叶绿素荧光仪而获得首届国际光合作用协会(ISPR)创新奖

    调制荧光成像系统 M系列IMAGING-PAM

    叶片被激光穿孔后NPQ信号的动态传递过程


    传统的光纤型调制荧光仪(如PAM-2100、MINI-PAM等)只能测量叶片上一个点的光合活性。利用一个点的数据代表一个叶片,利用一个叶片代表一个植株,进而代表一个群体(如森林、大田作物等),这种方法的误差是比较大的。

    从1980年代末期开始,科研人员就期望能通过成像型荧光仪来测量全叶片光合活性,并进行了不懈的尝试,但受技术上的限制,所设计的仪器无法商品化或商品化了但得不到大家认可。其中一个很重要的原因就是能够发出饱和脉冲水平强光的二极管(LED)尚未面世。要利用调制荧光技术测量全叶片水平的光合作用, 首先要保证叶片上任何一点所接受到的光强必须是完全相同的。调制荧光技术要求光源必须能发出很强的饱和脉冲光。 卤素灯能发出很强的光,但其光场非常不均匀,根本不能用于成像!装在一个平面上的LED阵列发出的光很均匀,但在2000年前,能发出超强光合有效辐射(PAR)的LED根本没有面世!

    2000年,能发出超强PAR的蓝光LED面世。2001年,全球最权威的调制荧光仪制造商德国WALZ公司设计制造了真正的全球第一台多功能调制荧光成像系统 IMAGING-PAMIMAGING-PAM采用超强发光LED作为光源,保证叶片表明受光均匀且光强足够强;IMAGING-PAM采用CCD作为检测器,能检测叶片上每个像素的光合作用;IMAGING-PAM秉承了WALZ公司PAM系列荧光仪的一贯优点,功能强大,测量参数多,操作极其简单,一面世就受到全球植物学家的青睐,迅速占领全球市场

    2005年,WALZ又推出了 M系列IMAGING-PAM,一个主机可以连接不同的探头(MICROSCOPY-,MICRO-,MINI-和MAXI-探头),分别在130×150 um、3.5×4.5 mm、24×32 mm或10×13 cm的面积上测量荧光成像。现在, 只需一个主机连接不同的探头,即可满足从单细胞到全叶片,从分子生物学到生态学研究的全面需要


    M系列IMAGING-PAM不同版本的比较


    MAXI-版

    MINI-版

    MICRO-版

    MICROSCOPY-版

           

    调制荧光成像系统 M系列IMAGING-PAM

    调制荧光成像系统 M系列IMAGING-PAM

    调制荧光成像系统 M系列IMAGING-PAM

    调制荧光成像系统 M系列IMAGING-PAM

           

    调制荧光成像系统 M系列IMAGING-PAM

    调制荧光成像系统 M系列IMAGING-PAM

    调制荧光成像系统 M系列IMAGING-PAM

    调制荧光成像系统 M系列IMAGING-PAM

           

    成像面积10×13 cm

    成像面积24×32 mm

    成像面积3.5×4.5 mm

    成像面积130×150 um

           

    放大1.5

    放大6

    放大45

    放大130-1300


    功 能
    * 一个主机连接不同的探头可满足从单细胞到全叶片、从分子生物学到生态学的不同需求
    * 全叶片光合作用分析(荧光成像),可测荧光诱导曲线并进行淬灭分析
    * 可测快速光响应曲线(120 s内完成,比光合放氧和气体交换等技术快得多)
    * 叶片光合作用的横向异质性检测
    * 完全相同的条件下同时测量多个样品(植物、地衣、苔藓、微藻等)
    * 遗传育种、突变株筛选的强大工具
    * 不同的测量面积,不同的分辨率
    * 可利用多孔板(如96孔板)做多个微藻样品的同时成像
    * 胁迫损伤的早期检测
    * 不连接显微镜即可测量绿色荧光蛋白(GFP)荧光
    * 可测量叶片吸光系数
    应用范围

    * 环境科学
    * 水生生物学
    * 海洋与湖沼学
    * 生态毒理学
    * 园艺学
    * 农业科学
    * 林学
    * 环境科学
    * 水生生物学
    * 海洋与湖沼学
    * 生态毒理学
    * 园艺学
    * 农业科学
    * 林学

    调制荧光成像系统 M系列IMAGING-PAM
    DCMU在叶片中的渗透过程


    测量参数
    调制荧光成像系统 M系列IMAGING-PAM

    * 以上所有参数均可成像
    * 吸光系数Abs和新参数qL、Y(NPQ)和Y(NO)的成像是IMAGING-PAM独有的
    * 生态毒理学研究中,选一个参考点,可以直接求出其它处理(如农药)的受抑制程度Inh.

    调制荧光成像系统 M系列IMAGING-PAM

    各种荧光参数的成像是将0.0(黑色)至1.0(紫色)的数值转换成颜色来显示的。


     


    M系列IMAGING-PAM不同版本介绍

     

     

     

    MAXI-版
    大探头,成像面积10×13 cm

    调制荧光成像系统的MAXI-探头利用300 W的LED阵列,可以在10×13 cm的面积上提供均为的调制测量光、光化光和饱和脉冲光。该探头的支架上配备特制护眼遮光罩,可以在保护眼睛的同时观测到红色荧光的变化。

    WALZ提供两种数码相机CCD供选择。用户若需要高清晰度,推荐选择IMAG-MAX/K[2/3” chip, 1392×1040象素, 4象素组合(binning)技术]。标准应用可选择IMAG-MAX/K2(1/2”, 640×480象素),可与IMAG-MAX/K2Z物镜(F1.0/f=8-48mm)结合使用。

    调制荧光成像系统 M系列IMAGING-PAM



    调制荧光成像系统 M系列IMAGING-PAM

    调制荧光成像系统 M系列IMAGING-PAM

    调制荧光成像系统 M系列IMAGING-PAM

    调制荧光成像系统 M系列IMAGING-PAM

    测量盆栽植物

    测量离体叶片

    测量微藻样品

    新增镜头可调放大倍数

           

    调制荧光成像系统 M系列IMAGING-PAM

    调制荧光成像系统 M系列IMAGING-PAM

    调制荧光成像系统 M系列IMAGING-PAM

    调制荧光成像系统 M系列IMAGING-PAM

    Y(NPQ)

    PS/50

    F

    96个微藻样品成像



    MINI-版
    小探头,成像面积24×32 mm

    调制荧光成像系统的MINI-探头采用强大的Luxeon LED阵列,包括4组(每组3个)LED,均配有长波截止滤光片。配备8个红光(650 nm)和8个近红外(780 nm)LED,用于测量叶片吸光系数的成像。

    3种版本可选
    IMAG-MIN/B:蓝光,450 nm,测量叶片等;
    IMAG-MIN/R:红光,620 nm,测量蓝藻
    IMAG-MIN/GFP:蓝光,480 nm,测量绿色荧光蛋白(GFP)

    调制荧光成像系统 M系列IMAGING-PAM

    由于MINI-探头的便携式设计,使其特别适合野外应用。由于MINI-探头的成像面积仅为MAXI-探头的1/16,因而前者发出的最大光强更大,但耗电却小得多。MINI-探头可以安装在光合仪GFS-3000的叶室3010-S上,同步测量全叶片气体交换和荧光成像,并且其光源可由GFS-3000控制,达到真正的同步测量
    MINI-探头采用1/3”数码相机CCD(640×480象素)和F1.2/f=12mm物镜。其设计目的为测量固定距离下的荧光成像。

    调制荧光成像系统 M系列IMAGING-PAM

    调制荧光成像系统 M系列IMAGING-PAM

    调制荧光成像系统 M系列IMAGING-PAM

    调制荧光成像系统 M系列IMAGING-PAM

    与光合仪GFS-3000连用

    长时间测量可装在三角架上

    Fm

    qN


    Micro-版
    微探头,成像面积3.5×4.5 mm

    调制荧光成像系统的MICRO-探头是一个极便携的探头,采用整合式Cosmicar-Pentax CCTV物镜(F1.4/f=16mm),直接安装在数码CCD(1/3” chip, 640×480像素)上。

    MICRO-探头只配备一个Luxeon LED(蓝光,450 nm)和一个特制双色分光镜,类似于落射荧光显微镜。

    尽管成像面积只有3.5×4.5 mm,但45倍的放大率却允许对叶片荧光成像的异质性分析达到支脉(minor veins)级。同时还可提供一个特制版本用于测量GFP的成像。

    调制荧光成像系统 M系列IMAGING-PAM


    MICRO-探头还可安装在标准版IMAGING-PAM(2001年设计)主机上。该探头提供X-Y轴可调的样品台。其设计目的为测量固定距离下的荧光成像。

    调制荧光成像系统 M系列IMAGING-PAM

    调制荧光成像系统 M系列IMAGING-PAM

    调制荧光成像系统 M系列IMAGING-PAM

    Fo

    GFP成像

    微探头直接安装在CCD上


    Microscopy-版
    显微探头,成像面积130×150 um

    必须与特制落射荧光显微镜(Hund或Zeiss)结合使用,该显微镜可以提供激发光并检测荧光

    IMAG-MAX/K(数码相机CCD)[1392×1040象素,4象素组合(binning)技术]可以提供高灵敏度。

    探头标准配置是一个超强Luxeon LED(450-480 nm),用于提供测量光、光化光和饱和脉冲。目前已可提供RGB探头(红-绿-蓝-白LED光源),它采用了PHYTO-PAM技术,可以显微镜下自动对蓝藻、绿藻、硅/甲藻、红藻进行分类并测量光合作用。

    调制荧光成像系统 M系列IMAGING-PAM


    调制荧光成像系统 M系列IMAGING-PAM

    调制荧光成像系统 M系列IMAGING-PAM

    分类,红色为硅藻,绿色为丝状绿藻

    光合,Fv/Fm活性,可区分细胞不同部位的活性


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